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KMID : 0381919730030010045
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Korean Journal of Microscopy
1973 Volume.3 No. 1 p.45 ~ p.54
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Electron Microscopic Observations of Mouse Liver Cell Treated with Fungal Culture Filtrates Isolated from Foodstuffs
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Abstract
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The present study is to determine the toxicity of the fungi isolated from foodstuffs by observing the ultra structural changes in the mouse liver cells.
The results as follows
1. The toxin-producing fungi were screened by the methods of toxin-screening test (cytotoxicity test against to HeLa cells and thin layer chromatography).
2. All of the experimental animals treated with isolated fungi were´ observed the focalnecrosis and inflammatory infiltration of liver parenchymal cells.
3. It showed the cytoplasmic changes,=such as dilatation of rough endoplasmic reticulum(RER), swelling of mitochondria- (mi), increased number of lipid droplet (li). and, glycogen(gl), detachment of ribosome’s(ri) by observing the electron microscopy.
4. Nuclear and nucleolar alteration were also noted the segregation of nucleolar element and irregularity of nuclear envelopes.
5. As a mass screening, the cytotoxicity test using HeLa cells and thin layer chromatography are feasible methods to detection of the mycotoxin producing fungi from various sources.
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KEYWORD
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